NGS-Solutions for Research & Health

Excellence in Genomics, Transcriptomics, Epigenetics and Bioinformatics

GenXPro GmbH was founded in 2005 by molecular biologists of the Universities of Frankfurt and Paris. Our team consists of molecular biologists, chemists and bioinformaticians dedicated to provide quality Next Generation Sequencing and qPCR solutions as well as research kits, software and data-analysis for quick and convenient access to the relevant information about DNA, DNA methylation, RNA and smallRNA.

We will help you unlock the information content of your sample- whether it is tissue, RNA, DNA, DNA methylation or raw NGS data.

We translate the data into accurate, easy to use formats. By comparing your results to the most significant databases we provide direct access to current state of knowledge available for your data, including diagnostically valuable information.

In 2008, we invented “TrueQuant” a technique to avoid PCR-introduced bias, a major problem of all sequence-based quantitative data. Besides the standard NGS techniques like RNA-Seq, smallRNA-Seq, Methyl-Seq we offer “MACE” (Massive Analysis of cDNA Ends) a powerful and cost-efficient technique that implies the “TrueQuant” method for the analysis of gene expression, alternative poly-Adenylation, allele-frequencies and for genotyping even of small amounts (e.g. from liquid biopsies) and degraded RNA, such as from FFPE samples.

We are constantly improving the sample preparation methods and bioinformatics pipelines to provide the best possible data from nucleotides of all kinds of tissues and body fluids including urine, blood and circulating cells.

Our customers are from all fields of life sciences. With a team of bioinformaticians and data scientists we design individual solutions and apply the most advanced software available for standard applications using our powerful CPU cluster. Our “know-how” is currently transferred into pipelines for precision medicine, to provide the best possible treatment in cancer, combining DNA and RNA-data and liquid biopsies, including information about the methylation of the DNA and other epigentic factors.

We are mourning the death of our friend, mentor, brilliant scientist and teacher Prof. Dr. Günter Kahl, who passed away in July 2015.

TrueQuant SmallRNA Seq Kit for Ultra Low Input, GEL FREE, Single Tube Protocol

The GenXPro small RNA sequencing kit (v1.0) can be used to prepare small RNA libraries to be sequenced on Illumina® NGS instruments starting with 0.01 to 500 ng of total RNA.

The library preparation methodology used in this kit allows an accurate identification and quantification of microRNAs, piRNAs and other small RNAs.

Fast & simple single Tube Protocol, Gel free!

The entire workflow takes place in a single tube. This minimizes losses and increases the efficiency tremendously. Even 0.01 ng of total RNA can be sufficient input material for library preparation.

Less PCR cycles, less duplicates: more output = much lower costs!

Thanks to the highly efficient method to generate a sequencable library from small RNA, usually between 5 and 14 PCR cycles less are needed to obtain the minimum amount needed to load a sequencing lane compared to other standard methods. Our kit therefore often allows to drastically reduce the sequencing costs of smallRNA between 50% and 80% compared to most other methods.

Automation

The single tube protocol strongly facilitates highly paralleled analyses of many samples on liquid handling systems.

TrueQuant Technology

GenXPro small RNA sequencing kit applies the proprietary TrueQuant Technology that eliminates PCR-derived copies from the generated gene expression data. Following sequencing of the adaptor-ligated and PCR-amplified libraries, only the sequence with highest quality of each TrueQuant adapter plus template-sequence combination are maintained.

Liquid Biopsies from only 200 µl of Plasma

Our protocol allows to generate smallRNA-Seq profiles from down to 200 µl of plasma.

High Technical Reliability and Sensitivity

Starting with DNase-treated and quality-controlled total RNA, adapters containing TrueQuant barcodes are ligated to both ends of the smallRNA, reverse transcribed and the final libraries are ready for sequencing on any of the Illumina MiniSeq, MiSeq, HiSeq, and NextSeq platforms. The present kit allows for multiplexing of up to 96 samples.

Trial possible!

Please send us an e-mail (info@genxpro.de) if you are interested in testing our smallRNA-kit.

Service:

We also offer regular and ultra-low input smallRNA seq service.

Price:

Kit for MiSeq, HiSeq or NextSeq (Illumina), Single End Sequencing

Name	Cat. Nr.
TQ-SmallRNA-Seq-6x	16047.8
TQ-SmallRNA-Seq-24x	16047.24
TQ-SmallRNA-Seq-48x	16047.48
TQ-SmallRNA-Seq-96x	16047.96

For orders or questions, please contact: info@genxpro.de

NEW PAPER:

Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA

Further References:

Wecker T, Hoffmeier K, Plötner A, Grüning BA, Horres R, Backofen R, Reinhard T, Schlunck G.MicroRNA Profiling in Aqueous Humor of Individual Human Eyes by Next-Generation Sequencing.Invest Ophthalmol Vis Sci. 2016 Apr 1;57(4):1706-1713.

Müller S, Raulefs S, Bruns P, Afonso-Grunz F, Plötner A, Thermann R, Jäger C, Schlitter AM, Kong B, Regel I, Roth WK, Rotter B, Hoffmeier K, Kahl G, Koch I, Theis FJ, Kleeff J, Winter P, Michalski CW. Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer. Mol Cancer. 2015 Apr 25;14(1):94.

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